Rise of Cobra (2009 Movie)
The ranks of Cobra Vipers originally consisted mostly of mercenaries and criminals (however after the completion of all of Cobra's nano experimentation, Neo-Vipers may have mostly consisted of kidnapped soldiers from around the world). 20 mercenaries were selected for the painful series of injections and the ones who didn't die from their pain were turned into the ultimate super soldiers.
Each subject was injected with 1,000 CCs of Nanomite solution which left a scar in the shape of a small cobra's head (all nano-injected members of Cobra bear this mark, this also includes Baroness, Zartan, and much later Destro). after injection specially designed nanomites both attack and rewire the subjects central nervous system making the subject completely under the absolute control of the Doctor (although the only exception to this was Zartan who had destroyed the neural controlling nanomites before he was injected).
Neo-Vipers consisted of the main ground force of Cobra's army. In one mission, the Cobra Vipers assisted Cobra agent Baroness in Cobra's attack on The Pit. Also, two Neo-Vipers piloted Cobra Commander's sub when he and James McCullen evacuated the M.A.R.S. Base, and the two were either killed or captured when Destro and Cobra Commander were captured by Duke and Ana.